cellaca mx (Revvity)

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Revvity cellaca mx
Cellaca Mx, supplied by Revvity, used in various techniques. Bioz Stars score: 95/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellaca+mx+cell+counter/Cellaca+MX+High-throughput+cell+counter/10__1186_slash_s41120___026___00207___w-55-11-13
Average 95 stars, based on 142 article reviews

cellaca mx - by Bioz Stars, 2026-10

95/100 stars

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Staining:

Article Title: Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity
Article Snippet: The Plasma Cell Isolation Kit II (Miltenyi Biotec) was then used to enrich total ASCs from BMMC samples following manufacturer’s instructions. .. A cell aliquot was stained using AO/PI viability stain and counted with the Nexcelom Cellaca MX cell counter. ..

Article Title: REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived Immunity
Article Snippet: .. The counting fraction was stained 1:1 with 27 μL of AO/PI solution and counted on a Nexcelom Cellaca MX cell counter. ..

Article Title: Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity
Article Snippet: BMMCs were subsequently enriched for total B cells by negative selection with the Human Pan-B Cell Enrichment Kit (Stemcell Technologies) following manufacturer’s instructions. .. A cell aliquot was stained with acridine orange/propidium iodide (AO/PI) viability dye and counted with the Nexcelom Cellaca MX cell counter. .. For mass cytometry assays, PBMC and BMMCs underwent magnetic lineage depletion using BD Streptavidin Particles Plus and the BD IMag Cell Separation Magnet (BD Biosciences) with a cocktail of biotinylated antibodies consisting of CD3, CD7, CD15, CD33, CD56, CD61, and CD235ab (BioLegend), according to the manufacturer’s instructions.

Cell Characterization:

Article Title: CAR-adapted PIK3CD base editing enhances T cell anti-tumor potency
Article Snippet: Then, 48 h after the last stimulation, living CAR T cells were single-cell-sorted (LNGFR + CD3 − ) and single-cell sequenced (CeGaT). .. Cell count and viability (>90%) were determined using a Cellaca MX cell counter (Revvity). .. After 18 h of incubation, 100 μl of luciferase substrate ( D -luciferin; Goldbio) was directly added to each well.

Concentration Assay:

Article Title: Multi-modal benchmarking of the Ultima UG100 and Illumina NovaSeq sequencing platforms using clinically relevant FFPE tissues
Article Snippet: .. Cell concentration was determined using the Cellaca MX cell counter (Revvity). .. 8,000 cells per sample were loaded onto a Chromium chip and processed using the Next GEM Single Cell Fixed RNA Reagent Kits (10x Genomics) according to the manufacturer’s protocol.

other:

Article Title: An NLRP3-stimulatory adjuvant improves the immunogenicity of influenza virus vaccines in mice and non-human primates
Article Snippet: Splenocytes were processed to single-cell suspension using Spleen Dissociation Kit (Miltenyi) and gentleMACS Tissue Dissociator (Miltenyi) set to program “37C_m_SDK_1.” After processing, spleens were treated with ACK lysing buffer (Gibco) and resuspended in R10 media.

Isolation:

Article Title: Rational modulation of immune mechanisms synergizes the anti-tumor effects of targeted radiation therapy in pre-clinical models
Article Snippet: Immune cells were isolated from cell suspensions using CD45 (TIL) MicroBeads, mouse (Miltenyi Biotec, San Diego, CA; Cat. No. 130-110-618) following manufacturer’s instructions. .. Isolated cells were counted using Cellaca MX Cell Counter (Nexcelom Bioscience, Lawrence, MA). ..

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