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cellaca mx cell counter  (Revvity)


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    Structured Review

    Revvity cellaca mx cell counter
    Cellaca Mx Cell Counter, supplied by Revvity, used in various techniques. Bioz Stars score: 95/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellaca+mx+cell+counter/Cellaca+MX+High-throughput+cell+counter/bio_rxiv__64898__2026__04__13__717827-346-26-25
    Average 95 stars, based on 141 article reviews
    cellaca mx cell counter - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity
    Article Snippet: The Plasma Cell Isolation Kit II (Miltenyi Biotec) was then used to enrich total ASCs from BMMC samples following manufacturer’s instructions. .. A cell aliquot was stained using AO/PI viability stain and counted with the Nexcelom Cellaca MX cell counter. ..

    Article Title: REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived Immunity
    Article Snippet: .. The counting fraction was stained 1:1 with 27 μL of AO/PI solution and counted on a Nexcelom Cellaca MX cell counter. ..

    Article Title: Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity
    Article Snippet: BMMCs were subsequently enriched for total B cells by negative selection with the Human Pan-B Cell Enrichment Kit (Stemcell Technologies) following manufacturer’s instructions. .. A cell aliquot was stained with acridine orange/propidium iodide (AO/PI) viability dye and counted with the Nexcelom Cellaca MX cell counter. .. For mass cytometry assays, PBMC and BMMCs underwent magnetic lineage depletion using BD Streptavidin Particles Plus and the BD IMag Cell Separation Magnet (BD Biosciences) with a cocktail of biotinylated antibodies consisting of CD3, CD7, CD15, CD33, CD56, CD61, and CD235ab (BioLegend), according to the manufacturer’s instructions.

    Article Title: Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity
    Article Snippet: Probe hybridization was completed according to the ‘Multiplexed samples with Feature Barcode technology for Protein using Barcode Oligo Capture’ protocol (10x Genomics, CG000673, Rev B) for 16-24 hours at 42°C. .. After probe hybridization, samples were diluted in Post-Hyb Wash Buffer, and a cell aliquot was stained with AO/PI viability dye and then counted with the Nexcelom Cellaca MX cell counter. .. Counted samples were then pooled at equivalent cell concentrations (when possible), incubated for 3 rounds of 10 minutes at 42°C, washed in Post-Hyb Wash Buffer, and strained using 30uM strainers (Miltenyi Biotec).

    Cell Characterization:

    Article Title: CAR-adapted PIK3CD base editing enhances T cell anti-tumor potency
    Article Snippet: Then, 48 h after the last stimulation, living CAR T cells were single-cell-sorted (LNGFR + CD3 − ) and single-cell sequenced (CeGaT). .. Cell count and viability (>90%) were determined using a Cellaca MX cell counter (Revvity). .. The scRNA-seq library was prepared using Chromium NextGEM single-cell 3′ kit, version 3.1 (10x Genomics) according to the manufacturer’s instructions.

    Concentration Assay:

    Article Title: Multi-modal benchmarking of the Ultima UG100 and Illumina NovaSeq sequencing platforms using clinically relevant FFPE tissues
    Article Snippet: .. Cell concentration was determined using the Cellaca MX cell counter (Revvity). .. 8,000 cells per sample were loaded onto a Chromium chip and processed using the Next GEM Single Cell Fixed RNA Reagent Kits (10x Genomics) according to the manufacturer’s protocol.

    Hybridization:

    Article Title: Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity
    Article Snippet: Probe hybridization was completed according to the ‘Multiplexed samples with Feature Barcode technology for Protein using Barcode Oligo Capture’ protocol (10x Genomics, CG000673, Rev B) for 16-24 hours at 42°C. .. After probe hybridization, samples were diluted in Post-Hyb Wash Buffer, and a cell aliquot was stained with AO/PI viability dye and then counted with the Nexcelom Cellaca MX cell counter. .. Counted samples were then pooled at equivalent cell concentrations (when possible), incubated for 3 rounds of 10 minutes at 42°C, washed in Post-Hyb Wash Buffer, and strained using 30uM strainers (Miltenyi Biotec).

    Isolation:

    Article Title: Rational modulation of immune mechanisms synergizes the anti-tumor effects of targeted radiation therapy in pre-clinical models
    Article Snippet: Immune cells were isolated from cell suspensions using CD45 (TIL) MicroBeads, mouse (Miltenyi Biotec, San Diego, CA; Cat. No. 130-110-618) following manufacturer’s instructions. .. Isolated cells were counted using Cellaca MX Cell Counter (Nexcelom Bioscience, Lawrence, MA). ..



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